Significance of Using Scanning Electron Microscopy with Energy Dispersive X-ray Spectrometry (SEM-EDX) for Analysis of Evidence Material in Forensic Sciences

Jumisree Sarmah Pathak *

Department of Physics, Indian Institute of Teacher Education (IITE), Gandhinagar, India.

Arvind Kumar Saxena

School of Forensic Science, National Forensic Sciences University (NFSU), Gandhinagar, India.

*Author to whom correspondence should be addressed.


Abstract

Forensic applications of Scanning Electron Microscopy (SEM) are found mostly in areas where there is a need for good imaging at relatively high magnifications. SEM enables the forensic scientist to examine specimens at much higher magnification than those possible with optical microscopy and without the difficulties of specimen preparation associated with conventional electron microscope.  Physicochemical examinations of gunshot residues, called also chemical ballistics, are helpful, e.g. in identification of damages and injuries as the effect of the use of firearms (with indicating the entrance and exit of projectile), estimation of the shooting distance and also establishing, whether a person has used a firearm. It is discussed in details that Scanning Electron Microscopy with Energy Dispersive X-Ray Spectroscopy (SEM-EDX) can serve as a powerful tool for forensic scientists to classify and discriminate evidence material because they can simultaneously examine the morphology and the elemental composition of objects.

Keywords: Gunshot residues, elemental analysis, scanning electron microscopy, forensic science


How to Cite

Pathak , J. S., & Saxena , A. K. (2024). Significance of Using Scanning Electron Microscopy with Energy Dispersive X-ray Spectrometry (SEM-EDX) for Analysis of Evidence Material in Forensic Sciences. Asian Journal of Research and Reviews in Physics, 8(2), 1–11. https://doi.org/10.9734/ajr2p/2024/v8i2159

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References

Scanning Electron Microscopy; 2024 Available:https://www.nanoscience.com/techniques/scanning-electron-microscopy/ accessed on 24th March, 2024.

Pilkington B. A Current View of Scanning Electron Microscopy in Forensics; 2022. Available:https://www.azom.com/article.aspx?ArticleID=21613

Taylor ME. Scanning Electron Microscopy in Forensic Science, J. Forens. Sc. Soc. 1973;13:269-280.

Vermeij E, Duvalois W, Webb R, Koeberg M. Morphology and composition of pyrotechnic residues formed at different levels of confinement, Forensic Science International. 2009;186(1–3):68-74.

Yashoda V, Munisekhar MS, Shylaja S, Rao KA, Reddy SK, Muddebihal F, Alam MK. An Ultrastructural Study on the Effect of High Temperatures on Teeth and Restorative Materials That Aids in the Identification of Human Remains. BioMed Research International. 2021;6629560.

Mummery D. Every contact leaves a trace. The British journal of general practice: The Journal of the Royal College of General Practitioners. 2021;71(712):512.

Zadora G, Brożek-Mucha Z. SEM–EDX—a useful tool for forensic examinations, Materials Chemistry and Physics. 2003;81(2–3):345-348.

Biancolillo A, Marini F Chemometric Methods for Spectroscopy-Based Pharmaceutical Analysis. Front Chem. 2018;21;6:576.

Han W, Jiao H, Fox D. Scanning Electron Microscopy. In: Wang R, Wang C, Zhang H, Tao J, Bai X. (eds) Progress in Nanoscale Characterization and Manipulation. Springer Tracts in Modern Physics, Springer, Singapore. 2018;272

Scimeca M, Bischetti S, Lamsira HK, Bonfiglio R, Bonanno E. Energy Dispersive X-ray (EDX) microanalysis: A powerful tool in biomedical research and diagnosis. Eur J Histochem. 2018;62(1):2841.

Ward DC. Gunshot residue collection for scanning electron-microscopy. Scan Electron Micros. 1982;3:1031–1036.

French J, Morgan R, Davy J. The secondary transfer of gunshot residue: An experimental investigation carried out with SEM-EDX analysis. X-Ray Spectrometry. 2013;43:56-61

Gunaratnam L, Himberg K. The Identification of Gunshot Residue particles from Leadfree Sintox Ammunition. Journal of Forensic Sciences. 1994;39:532–536.

Brożek-Mucha Z. Scanning electron microscopy and X-ray microanalysis for chemical and morphological characterisation of the inorganic component of gunshot residue: Selected problems. BioMed Research International. 2014;428038.

Taudte RV, Beavis A, Blanes L, Cole N, Doble P, Roux C.Detection of gunshot residues using mass spectrometry. BioMed Research International; 2014.

DeGaetano D, Siegel JA. Survey of gunshot residue analysis in forensic science laboratory. Journal of Forensic Sciences. 1990;35:1087-1095.

Romolo FS, Margot P. Identification of gunshot residue: A critical review. Forensic Science International. 2001;119(2):195-211.

Hellmiss G, Lichtenberg W, Weiss M. Investigation of gunshot residues by means of auger electron spectroscopy. J Forensic Sci. 1987;32(3):747–760

Streli C, Wobrauschek P, Kregsamer P X-Ray Fluorescence Spectroscopy, Applications, Editor(s): John C. Lindon, George E. Tranter, David W. Koppenaal, Encyclopedia of Spectroscopy and Spectrometry (Third Edition), Academic Press. 2017;707-715

Badla C, Wewers F. Optimization of X-ray Fluorescence Calibration through the Introduction of Synthetic Standards for the Determination of Mineral Sands Oxides, S. Afr. J. Chem. 2020;73:92-102.

Muratsu S, Ninomiya T, Kagoshima Yi, Matsui J. Trace Elemental Analysis of Drugs of Abuse Using Synchrotron Radiation Total Reflection X-Ray Fluorescence Analysis (SR-TXRF). Journal of Forensic Sciences. 2002;47:944-949.

Gallusser A, Bonfanti M, Schu¨tz F. Expertise des armes a feu et des elements de munition dans l'investigation criminelle (1ere ed.). Lausanne: Presses polytechniques et universitaires romandes. 2002;129-152.

Kersh KL, Childers JM, Justice D, Greg K. Detection of gunshot residue on dark-colored clothing prior to chemical analysis. Journal of Forensic Sciences. 2014;59(3):754-762.

Basu S. Formation of gunshot residues. Journal of Forensic Sciences. 1982;27:72-91.

Mosher PV, McVicar MJ, Randall ED, Su EH. Gunshot residue similar particles produce fireworks. Canadian Society of Forensic Science Journal. 1998;31(2):157-168.

Chohra M, Beladel B, Ahmed LB, Mouzai M, Akretche D, Zeghdaoui A, Mansouri A, Benamar MEA. Study of gunshot residue by NAA and ESEM/EDX using several kinds of weapon and ammunition. Journal of Radiation Research and Applied Sciences. 2015;8:404-410.

Lebiedzik J, Johnson DL. Rapid search and quantitative analysis of gunshot residue particles in the SEM. J Forensic Sci. 2000;45(1):83–92.

Collins P, Coumbaros J, Horsley G, Lynch B, Kirkbride KP, Skinner W. Glass-containing gunshot residue particles: A new type of highly characteristic particle. J Forensic Sci. 2003;48(3):538–553.

Forensic Paint Analysis and Comparison Guidelines, Scientific Working Group on Materials Analysis (SWGMAT), Paint Subgroup; 2000 Available:https://www.asteetrace.org/static/images/pdf/01%20Forensic%20Paint%20Analysis%20and%20Comparison%20Guidelines.pdf

Malek MA, Nakazawa T, Kang HW, Tsuji K, Ro CU. Multi-Modal Compositional Analysis of Layered Paint Chips of Automobiles by the Combined Application of ATR-FTIR Imaging, Raman Microspectrometry, and SEM/EDX. Molecules (Basel, Switzerland). 2019;24(7):1381.

Lavine BK, Fasasi A, Mirjankar N, White C, Sandercock M. Search prefilters to assist in library searching of infrared spectra of automotive clear coats, Talanta. 2015;132:182-190.

Kaur N, Abi KS, Verma P, Mahajan M. An elemental analysis of Indian automotive paint using SEM-EDS. Materials Today: Proceedings. 2022;48(5):1748-1753.

Zieba-Palus J, Aleksandra M, Wesełucha-Birczyńska A. Characterisation of paint samples by infrared and Raman spectroscopy for criminalistic purposes. Journal of Molecular Structure. 2011;993(1–3):134-141.

Franquelo ML, Duran A, Herrera LK, Jimenez de Haro MC, Perez-Rodriguez JL. Comparison between micro-Raman and micro-FTIR spectroscopy techniques for the characterization of pigments from Southern Spain Cultural Heritage. Journal of Molecular Structure. 2009;924–926:404-412.

Elemental Analysis of Glass. Guidelines by Scientific Group for Working of Materials Analysis (SWGMAT); 2004 Available:https://www.asteetrace.org/static/images/pdf/05%20Elemental%20Analysis%20of%20Glass.pdf

Khan T. Elemental Analysis of Glass Using SEM, AZO Materials; 2022 Available:https://www.azom.com/article.aspx?ArticleID=21434

Parker B, Peterson J, Report of the National Institute of Law Enforcement and Criminal Justice, Washington, DC; 1972. Available:https://www.academia.edu/28162434/Elemental_analysis_of_small_glass_fragments_in_forensic_science

Buscaglia J. Elemental analysis of small glass fragments in forensic science, Analytica Chimica Acta. 1994;288(1–2):17-24.

Almirall J, Naes B, Cahoon E, Trejos T., Elemental Analysis of Glass by SEM-EDS, μXRF, LIBS and LA-ICP-MS, Final Technical Report: 2005-IJ-CX-K069 submitted to U.S. Department of Justice; 2012. Available:https://www.ojp.gov/pdffiles1/nij/grants/240592.pdf

Ryland S. Sheet or container? — Forensic glass comparisons with an emphasis on source classification. Journal of Forensic Sciences. 1986;31:1314-1329.

Terry KW, Van Riessen A, Vowles DJ. Elemental analysis of glasses in a SEM, Micron. 1982;13:293-294.

Keeley RH, Christofides S. Classification of small glass fragments by X-ray microanalysis with the SEM and a small sample XRF spectrometer. In: Proceedings of Scanning Electron Microscopy, SEM, AMF O'Hare, Illinois, Part I. 1979;459-464.

Andrasko J, Maehly AC. The discrimination between samples of window glass by combining physical and chemical techniques. Journal of Forensic Sciences. 1978;23:250-262.

Mershon J. Forensic Analysis of Mobile Phone Glass with SEM, EDS and Micro-CT. Forensic On the Scene and In the Lab; 2021. Available:https://www.forensicmag.com/576948-Forensic-Analysis-of-Mobile-Phone-Glass-with-SEM-EDS-and-Micro-CT/

Automated Gunshot Residue Analysis Using Scanning Electron Microscopy, Technologies: Scanning Electron Microscopy, Nanoscience Instruments; 2023 Available:www.nanoscience.com/applications/automated-gunshot-residue-analysis-using-scanning-electron-microscopy/

Standard Practice for Gunshot Residue Analysis by Scanning Electron Microscopy/ Energy Dispersive X-Ray Spectrometry, Gunshot Residue Subcommittee Chemistry Scientific Area Committee Organization of Scientific Area Committees (OSAC) for Forensic Science; 2020 Available:https://www.scribd.com/document/703507438/Osac-Gsr-Sc-e1588-for-Sac

Using SEM to Accelerate Gunshot Reside Analysis, AZO Materials; 2019 Available:https://www.azom.com/article.aspx?ArticleID=18158

Von Ardenne M, Hawkes P, Mulvey T. Chapter Two - On the history of scanning electron microscopy, of the electron microprobe, and of early contributions to transmission electron microscopy. Eds: Hawkes, P.W. and Hÿtch, M. Advances in Imaging and Electron Physics, Elsevier. 2021;220:25-50.

Wolten GM, Nesbitt RS, Calloway AR, Loper GL, Jones PF. Particle analysis for the detection of gunshot residue. I: Scanning electron microscopy/energy dispersive X Ray characterisation of hand deposits from firing. Journal of Forensic Sciences. 1979;24:409-422.